HEX Filter Set For PCR
HEX filter set is a fluorescence filter set designed for HEX detection in PCR. Coligh manufacture high quality hex fluorescence filter set and many other PCR fluorescence filter set.
- Exitation filter 530/20nm, emission filter 565/20nm, dichroic filter 550nm
- Blocking depth OD>6
- Transmission>90%
- Compatible with VIC, JOE and Tamra fluorescent dyes
- Custom wavelength and filter size are available
Products Categories
Get A Free Quote
HEX PCR Filter Set Description
The HEX filter set produced by Coligh is specially designed for PCR.
- The design of excitation peak 530nm, emission peak 560nm and narrow bandwidth 20nm can closely match HEX dyes and maximize the capture of fluorescence signals
- The dichroic mirror 550nm can efficiently separate excitation light and emission light, minimizing light path crossover
- The cutoff depth of OD>6 can suppress the interference of laboratory lights and stray light, improve the signal-to-noise ratio, and enhance the detection of extremely weak fluorescence signals
- This filter set can also be used to adapt to other fluorescent dyes, such as VIC, JOE and Tamra, and can be expanded to qPCR and high-pass sequencing
The hard coating process is used, with little wavelength drift and extremely high consistency to ensure stable results of experimental batches
HEX Fluorescence Filter Set For PCR Spectrum
HEX Fluorescence Filter Set Datasheet
| Optical Filter type: | Fluorescence Filter Set | Fluorophore Detection: | HEX/JOE/VIC/Tamra |
| Exitation Filter: | 530/20nm | Emission Filter: | 565/20nm |
| Dichroic Filter: | 550nm | Transmission: | >90% |
| Blocking Depth: | >0D6 | Blocking Range: | 200-1100nm |
| Angle of Incidence: | 0 deg for EX and EM filter.
45 deg for dichroic filter |
Diameter avaliable | Dia12, Dia12.7, Dia18, Dia25, Dia25.4 |
| Surface quality: | 60/40 | Thickness: | 1mm unmounted, 6mm mounted availiable |
HEX Fluorescence Filter Set Applications
The HEX filter combination has the following main applications:
- PCR
In qPCR and multiplex fluorescence detection qPCR experiments, the HEX filter combination is used to select HEX-labeled fluorescent dyes, collect HEX emission signals, distinguish different fluorescent signals, and avoid interference from other signals - Multiplex fluorescence labeling imaging
In multiplex fluorescence labeling experiments, researchers need to detect signals from different fluorescent dyes. The HEX filter set can accurately filter and analyze signals from different dyes so that the signals do not interfere with each other - Fluorescence resonance energy transfer technology
FRET can detect the interaction between two fluorescently labeled molecules. The HEX filter set can help distinguish the emission light of the donor and the acceptor and capture the FRET signal - Flow cytometer The HEX filter set can be used to analyze the characteristics of individual cells and accurately select the wavelengths used for excitation and detection
