Cy5 Filter Set For PCR
Cy5 filter set is specially designed for PCR Cy5 detection. Coligh manufactures high performance Cy5 fluorescence filter sets for PCR applications.
- Cy5 exitation filter 630/20nm Emission filter 665/20nm Dichroic filter 590nm
- High blocking depth 200-1100nm@OD>6
- Transmission >90%
- Cy5 filter set option 2: EX620/16nm EM665/16nm DM635nm
- Cy5 filter set option 3: EX635/20nm EM682/20nm DM662nm
- Cy5 filter set option 4: EX640/16nm EM680/16nm DM662nm
Cy5 PCR Filter Set Desription
Coligh provides professional PCR detection filter sets for PCR detection. The following four filter combinations are specially designed for Cy5 fluorescence signal detection to ensure accurate data.
- EX630/20 EM665/20 DM590 This filter set is suitable for efficient red light detection. In qPCR detection, it can detect Cy5 Alexa Fluor 647 fluorescent dyes
- EX620/16 EM665/16 DM635nm This filter set has a relatively long distance between the excitation light and the emission light. It belongs to the red light channel with low crosstalk and is suitable for qPCR and fluorescent protein markers, such as Dylight 633 and Alexa Fluor 633
- EX635/20nm EM682/20nm DM662nm is suitable for far-red fluorescent dyes, such as Alexa Fluor 647, Cy5, and DyLight 650, ensuring accurate fluorescence detection and improving experimental repeatability.
- EX640/16 EM680/16 DM662nm, suitable for far-red fluorescence detection, such as Alexa Fluor 680, IRDye 680.
These filters have the following features：
- >90% transmittance can maximize the target fluorescence signal
200-1100nm@OD>6 blocking can accurately block non-target wavelengths and effectively reduce background interference - The narrow bandwidth design of 16nm/20nm can ensure that there is no interference between different fluorescence signals, suitable for multiple fluorescence experiments of qPCR
- The use of hard coating technology can ensure that the optical performance does not decay for a long time
Cy5 Fluorescence Filter Set Spectrum Performance
Cy5 Filter Set Technical Datasheet
| Optical Filter type: | Fluorescence Filter Set | Fluorophore Detection: | Cy5、Quasar® 670 |
| Exitation Filter: | 620/16nm
630/20nm 635/20nm 640/16nm |
Emission Filter: | 665/16nm
665/20nm 682/20nm 680/16nm |
| Dichroic Filter: | DM635nm、DM662nm、DM647nm | Transmission: | >90% |
| Blocking Depth: | >0D6 | Blocking Range: | 200-1100nm |
| Angle of Incidence: | 0 deg for EX and EM filter.
45 deg for dichroic filter |
Diameter avaliable | Dia12, Dia12.7, Dia18, Dia25, Dia25.4 or custom |
| Surface quality: | 60/40 | Thickness: | 1mm unmounted, 6mm mounted availiable |
Cy5 Fluorescence Filter Set Applications
The Cy5 fluorescence filter set produced by Coligh is suitable for the following applications:
- Fluorescence quantitative PCR
These filters are suitable for far-red fluorescent dyes, and can ensure accurate fluorescence detection in fluorescent dyes such as Alexa Flor 647, Cy5, Dylight 650
- Fluorescent protein and nucleic acid detection
In fluorescent protein labeling and RNA/DNA analysis, high-sensitivity signal detection and recognition capabilities can be achieved
- Fluorescence imaging and cell analysis
When used in cell fluorescence imaging, FISH and other scenarios, the contrast of fluorescent signals can be improved
- Molecular diagnosis and in vitro testing
In the fields of pathogen detection, genetic disease screening, etc., it can assist in achieving diagnostic accuracy
