Cy5.5 Filter Set For PCR
Cy5.5 filter set is a high performance fluorescence filter set specially designed for far-red fluorescent dye Cy5.5. Coligh manufactures high quality hard coating interference filter set for PCR application
We provide two filter sets of filter sets for Cy5.5 detection.
- Set 1: EX675/20nm EM725/40nm DM702nm
- Set 2: EX682/20nm EM725/40nm DM702nm
- Cut-off depth: 200-1100nm@OD>6, reduce autofluorescence and improve data reliability
- Transmittance>90%, maximize fluorescence signal transmission
- High steepness filter design to reduce interference from non-target wavelengths
Cy5.5 Filter Set for PCR Description
COligh provides two filter sets for Cy5.5 fluorescence detection, which accurately cover the excitation and emission spectra of Cy5.5 (excitation spectrum 675-682nm emission spectrum 710-750nm), ensuring high sensitivity of fluorescence signal detection
- Set 1 (EX675/20 – EM725/40 – DM702)
- Set 2 (EX682/20 – EM725/40 – DM702)
Both can improve the excitation efficiency of Cy5.5 dye and optimize the quality of far-red fluorescence signal
In these optical filter sets, the transmittance of the filter is >90%, and the ultra-strong cutoff depth of 200-1100nm@OD>6 can block the interference of background light, maximize the transmission of fluorescence signal, and improve the reliability of data. Before the production of the filter, we design high-steepness filtering to improve the purity of the target fluorescence signal.
In addition, we use hard coating technology, which can be corrosion-resistant and high-temperature-resistant and can be used for a long time
Cy5.5 Filter Set Spectrum Performance
Cy5.5 Filter Set Technical Datasheet
| Optical Filter type: | Fluorescence Filter Set | Fluorophore Detection: | Cy5.5, Quasar® 705 |
| Exitation Filter: | 682/15nm
675/20nm |
Emission Filter: | 725/40nm |
| Dichroic Filter: | DM702 | Transmission: | >90% |
| Blocking Depth: | >0D6 | Blocking Range: | 200-1100nm |
| Angle of Incidence: | 0 deg for EX and EM filter.
45 deg for dichroic filter |
Diameter avaliable | Dia12, Dia12.7, Dia18, Dia25, Dia25.4 or custom |
| Surface quality: | 60/40 | Thickness: | 1mm unmounted, 6mm mounted availiable |
Cy5.5 PCR Filter Set Applications
The Cy5.5 fluorescent filter set is used in many high-end scientific research and clinical testing fields. The following are the main application areas:
- In small animal in vivo imaging, tissue imaging and fluorescent labeling, the Cy5.5 signal can penetrate deeper tissues and is used in cancer research and immune research.
- In new disease detection and tumor gene detection, the Cy5.5 fluorescent probe can provide a highly sensitive detection solution
- The Cy5.5 fluorescent filter set can be used for fluorescent immunoassay and liquid biopsy for early cancer screening
Through fluorescent PCR, the Cy5.5 filter set can be used for the detection of new crown, AIDS and tuberculosis
