Cy3 Filter Set for PCR
Cy3 filter set for PCR is an optical fluorescence filter set for detecting Cy3 in PCR. Coligh supplys high quality Cy3 filter sets and many others for PCR instrument
- Cy3 exitation 543/20nm Emission 585/20nm Dichroic filter 565
- Blocking depth 200-1100nm@OD>6
- Transmission >90%
- Cy3 filter set option 2 are also avaliable: exitation filter 545/25nm emission filter 585/20nm dichroic filter 565nm
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Cy3 Optical Fluorescence Filter Set Description
Coligh’s Cy3 filter set is specially designed for PCR detection of Cy3 fluorescent dyes. It has the following features:
- The wavelength center wavelength of the excitation filter is 550nm and the wavelength center wavelength of the emission filter is 570nm, which is highly overlapped with the excitation peak and emission peak of Cy3, and maintains an extremely high transmittance of >90%, which can maximize the intensity of the Cy3 fluorescent signal
- The wavelength of the dichroic mirror is 570nm, which can effectively separate the excitation light and the emission light to avoid signal crosstalk caused by spectral overlap
- The excitation filter and the emission filter have a cutoff depth of OD>6, which can effectively filter the ambient stray light and reduce background noise
- Cy3 PCR filter is compatible with most PCR timing and quantitative instruments and can be integrated into the existing PCR detection system
- Coligh is specialized in customizing various fluorescent filters. You are welcome to submit customized requirements of various wavelengths and sizes
Cy3 Optical Fluorescence Filter For PCR Detection Spectrum
Cy3 Optical Filter Set Datasheet
| Optical Filter type: | Fluorescence Filter Set | Fluorophore Detection: | Cy3 |
| Exitation Filter: | 543/20nm | Emission Filter: | 585/20nm |
| Dichroic Filter: | 565nm | Transmission: | >90% |
| Blocking Depth: | >0D6 | Blocking Range: | 200-1100nm |
| Angle of Incidence: | 0 deg for EX and EM filter.
45 deg for dichroic filter |
Diameter avaliable | Dia12, Dia12.7, Dia18, Dia25, Dia25.4 or custom |
| Surface quality: | 60/40 | Thickness: | 1mm unmounted, 6mm mounted availiable |
Cy3 Filter Set For PCR Applications
Cy3 is a common red fluorescent dye, suitable for a variety of biological experiments. The main applications of Cy3 PCR filters are as follows:
- Multiple real-time PCR and fluorescence quantitative analysis
Cy3 filters, together with other fluorescent dyes, can accurately capture fluorescent signals through narrow-band filters and detect multiple gene targets at the same time. - Quantitative analysis of gene expression
In real-time quantitative PCR, by detecting changes in Cy3 fluorescent signals, researchers can obtain highly sensitive gene expression data and analyze multiple genes - DNA mutation and DNA array analysis
In gene mutation detection and DNA array detection, Cy3 filters can detect tiny DNA mutations and DNA detection - RNA sequencing By labeling cell surface markers with Cy3, single cell sorting and counting can be achieved
