FAM Filter Set For PCR
FAM Filter Set For PCR is a fluoresscence filter set consist of exitation filter 470/30nm, emission filter 520/20nm and dichroic mirror 505nm. Coligh manufactures premium high quality optical filter for PCR system.
- Excitation fluorescence filter 470/30nm and emission filter 520/20nm
- Transmittance and reflectance over 95%
- Extremely deep cut-off depth OD>6
- Comply with MIL-STD-810F, MIL-C-48497A standards
- FAM filter set option 2: exitation filter 570/30nm Emission filter 525/20nm and dichroic filter 505nm filter also avaliable
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FAM Optical Fluorescence Filter Set For PCR Description
Coligh provides high-quality optical filters for detecting FAM/SYBR Green fluorophores.
- The excitation filter 470/30nm and the emission filter 520/20nm are specially designed for FAM and SYBR Green fluorophores (excitation peak 470nm, emission peak 520nm). The precise narrow bandwidth design ensures efficient separation of excitation light and fluorescence signals.
- Extremely deep cutoff depth OD>6: ensures strong signal blocking ability, effectively removes unwanted wavelengths, and enhances signal purity. OD>6 (≥99.999% light attenuation): achieves super strong light suppression ability in non-target wavelength areas (such as around 500nm), completely eliminates cross-signal interference, and ensures low-abundance sample detection sensitivity.
- Coligh’s fluorescence filters meet MIL-STD-810F, MIL-C-48497A standards, and are resistant to vibration, temperature cycling and high humidity, meeting the stringent requirements of laboratory and field testing.
PCR Optical Fluorescence Filter For FAM and Sybr Green Fluorephore
FAM Fluorescence Filter Set Technical Datasheet
| Optical Filter type: | Fluorescence Filter Set | Fluorophore Detection: | FAM/SybrGreen |
| Exitation Filter: | 470/30nm | Emission Filter: | 520/20nm |
| Dichroic Filter: | 505nm | Transmission: | >90% |
| Blocking Depth: | >0D6 | Blocking Range: | 200-1100nm |
| Angle of Incidence: | 0 deg for EX and EM filter.
45 deg for dichroic filter |
Diameter avaliable | Dia12, Dia12.7, Dia18, Dia25, Dia25.4 |
| Surface quality: | 60/40 | Thickness: | 1mm unmounted, 6mm mounted availiable |
FAM Filter Set Applications
FAM fluorescence filter set is mainly used for fluorescence imaging and analysis, such as molecular detection, cell analysis and clinical diagnosis. Its applications are mainly reflected in:
- PCR and qPCR DNA sequencing and microarray analysis for precision medical research
- In flow cytometry, FAM green fluorescence is suitable for cell surface detection, protein localization, and multicolor experiment optimization
- In fluorescence microscope imaging, FAM fluorescence filters can be used for immunofluorescence staining, fluorescence in situ hybridization and cell dynamic tracking
- In biochemistry, FAM fluorescence filters can be used to track enzyme activity (such as Caspase detection) or ion concentration changes (requires photostability optimization)
